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Involvement of acetosyringone in plant-pathogen recognition
C Jacyn Baker1, Norton M Mock, Bruce D Whitaker
1Molecular Plant Pathology Laboratory, ARS, USDA, Beltsville, MD 20705, USA. bakerc@ba.ars.usda.gov
Biochemical and Biophysical Research Communications
|January 27, 2005
Summary
Acetosyringone, a plant phenolic, accelerates oxygen bursts in resistant tobacco-bacterial interactions. This bioactive compound influences plant-pathogen defense mechanisms, highlighting its role in plant-bacterial pathogenesis.
Area of Science:
- Plant Pathology
- Plant-Microbe Interactions
- Biochemistry
Background:
- Acetosyringone is a major extracellular phenolic in tobacco suspension cells.
- Plant-bacterial pathogenesis involves complex signaling events.
- Extracellular phenolics may play roles in plant defense responses.
Purpose of the Study:
- To investigate the bioactive properties of acetosyringone in plant-bacterial pathogenesis.
- To determine the effect of exogenous acetosyringone on tobacco cell suspension responses to bacterial isolates.
Main Methods:
- Tobacco cell suspensions were treated with Pseudomonas syringae WT (HR+) and Pseudomonas tabaci.
- Extracellular acetosyringone concentrations were supplemented exogenously.
- Oxygen uptake bursts and oxidative degradation of acetosyringone were monitored.
Main Results:
- Exogenous acetosyringone accelerated the oxidative burst in resistant interactions with P. syringae WT (HR+) by up to 1.5 hours.
- Acetosyringone supplementation had no effect on susceptible interactions with P. tabaci or non-resistant interactions.
- Resistant interactions with P. syringae WT (HR+) led to oxidative degradation of extracellular acetosyringone.
Conclusions:
- Acetosyringone exhibits bioactive properties influencing early plant-bacterial pathogenesis events.
- The compound specifically modulates resistant plant-bacterial interactions.
- Extracellular phenolics, including acetosyringone, are likely key players in plant defense signaling.