Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

COPII coat assembly and selective export from the endoplasmic reticulum.

Ken Sato1

  • 1Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351-0198, Japan. kensato@riken.jp

Journal of Biochemistry
|January 27, 2005
PubMed
Summary

The Sar1 GTPase cycle regulates coat protein complex II (COPII) vesicle formation. Cargo proteins may influence Sar1 GTP hydrolysis, coordinating vesicle assembly with cargo selection during ER transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temporal and spatial RNA expression dynamics in postmortem skin support PMI estimation and reveal spatial signatures of antemortem injury.

International journal of legal medicine·2026
Same author

Enhancing virtual bronchoscopic navigation route to small peripheral pulmonary lesions using lateral decubitus computed tomography with ultrathin bronchoscope: A single-center retrospective study.

Respiratory investigation·2026
Same author

Marked Respiratory Improvement After ECMO-Supported Exchange From Metallic to Silicone Stent in Tracheobronchial Stenosis due to Tuberculosis: A Case Report.

Respirology case reports·2026
Same author

Evaluating the value and risks of DNA evidence in Japanese criminal trials: Changes before and after the lay judge system.

Legal medicine (Tokyo, Japan)·2026
Same author

Germ granule components regulate endosomal switching during the oocyte-to-embryo transition in Caenorhabditis elegans.

Communications biology·2026
Same author

Transmembrane ROOM proteins ensure rooms for germ cells by maintaining intercellular bridges.

Proceedings of the National Academy of Sciences of the United States of America·2026

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Coat protein complex II (COPII) mediates essential protein transport from the endoplasmic reticulum (ER).
  • COPII coat assembly and vesicle formation are initiated by the Sar1 GTPase cycle.
  • Previous studies highlight COPII's role in direct cargo capture and ER membrane deformation.

Purpose of the Study:

  • To explore the function of Sar1 GTP hydrolysis in COPII coat assembly.
  • To investigate the coordination between coat assembly and cargo selection.
  • To elucidate the role of cargo proteins in modulating Sar1 GTPase activity.

Main Methods:

  • Literature review of recent structural and biochemical studies.
  • Analysis of the Sar1 GTPase cycle in vesicle formation.

Related Experiment Videos

  • Discussion of emerging evidence on cargo-mediated regulation.

    • Main Results:

    • Sar1 GTPase activation triggers COPII coat assembly.
    • Sar1 GTP hydrolysis leads to coat disassembly and protein recycling.
    • Cargo proteins may modulate Sar1 GTP hydrolysis to link coat assembly and cargo selection.

    Conclusions:

    • The Sar1 GTPase cycle is central to COPII vesicle biogenesis.
    • Cargo proteins play a potential regulatory role in ER-to-Golgi transport.
    • Understanding Sar1 function is key to comprehending vesicle trafficking mechanisms.