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A new two-chip concept for continuous measurements on PMMA-microchips.

Oliver Vogt¹, Markus Pfister, Ulrich Marggraf

¹Institute for Analytical Sciences, P.O. Box 101352, D-44013 Dortmund, Germany. jacob@ansci.de

|January 27, 2005

Summary

A novel polymethylmethacrylate capillary electrophoresis chip system enables continuous, automated microchip analyses. This two-chip design offers stable, reproducible measurements of small ions with minimal user intervention.

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Area of Science:

Analytical Chemistry
Microfluidics
Separation Science

Background:

Continuous analysis on microchips is challenging due to stability and reproducibility issues.
Existing microchip systems often require frequent refilling, cleaning, or chip replacement.

Purpose of the Study:

To present a new concept for continuous measurements on microchips.
To develop a stable and reproducible two-chip system for long-term analyses.

Main Methods:

A polymethylmethacrylate (PMMA) based capillary electrophoresis chip with integrated conductivity detection was combined with a fluid handling and electrical connection chip.
The system utilized a combination of electrokinetic and hydrodynamic flows.
Characterization focused on stability and reproducibility of separation and detection of small ions.

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Main Results:

The two-chip system achieved long-term continuous stable analyses.
Good reproducibility of migration time (<1% RSD) and peak heights (3% RSD) for analytes was demonstrated.
The system allowed automated analysis without the need for refilling or cleaning.

Conclusions:

The presented two-chip system offers a robust solution for continuous microchip-based analyses.
It simplifies handling and automation, reducing the need for manual intervention.
This approach enhances the practicality of microchip electrophoresis for routine measurements.