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Updated: Aug 19, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1
Yuji Kamikubo1, Naoko Sakaguchi, Keiji Shikata
1Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Abstract:
Importin alpha1/Rch1, an importin alpha family member, mediates the nuclear import of karyophilic proteins. The present study reports on a monoclonal antibody (MAb) directed against mammalian importin alpha1/Rch1, which was produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The MAb 1A6 specifically recognized importin alpha1/Rch1 among the importin alpha isoforms, as evidenced by Western blotting. Furthermore, 1A6 detected importin alpha1/Rch1 in HeLa cells by immunofluorescence. This MAb will be useful in immunolocalization and immunoblotting experiments, conducted on different tissue types, to determine the levels of expression of importin alpha1/Rch1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.
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