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Ubiquitous GFP expression in transgenic chickens using a lentiviral vector
Susan C Chapman1, Aaron Lawson, William C Macarthur
1University of Utah School of Medicine, Department of Neurobiology and Anatomy, and Children's Health Research Center, Room 401 MREB, 20 North 1900 East Salt Lake City, Utah 84132-3401, USA. susan.chapman@utah.edu
Development (Cambridge, England)
|January 28, 2005
Summary
Researchers achieved ubiquitous green fluorescent protein expression in chicks using lentiviral vectors. This breakthrough enables a new transgenic chick model for developmental studies and pharmaceutical applications.
Area of Science:
- Genetics and Developmental Biology
- Biotechnology
- Molecular Biology
Background:
- Previous chick transgenesis relied on tissue-specific markers, limiting applications.
- Ubiquitous expression is crucial for comprehensive developmental studies and broader biotechnological uses.
Purpose of the Study:
- To establish a transgenic chick model with ubiquitous enhanced green fluorescent protein (eGFP) expression.
- To demonstrate germline transmission and utility of this new model.
Main Methods:
- Utilized a lentiviral vector approach for gene delivery.
- Employed the phosphoglycerol kinase promoter to drive ubiquitous eGFP expression.
- Verified germline transmission and stable integration of the transgene.
Main Results:
- Achieved the first ubiquitous eGFP expression in chicks.
- Demonstrated uniform eGFP expression in embryonic tissues.
- Confirmed germline transmission and the ability to breed homozygous transgenic chicks.
- Transgenic tissue is identifiable via fluorescence microscopy and immunolabeling.
Conclusions:
- A viable and useful transgenic chick model with ubiquitous expression has been developed.
- This model facilitates studies in embryonic development and offers potential as a bioreactor.
- The long-term potential for producing transgenic embryos in 100% of eggs from homozygous lines is established.