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Related Experiment Videos

Evidence that disinhibition is associated with a decrease in number of vesicles available for release at inhibitory

Veronica A Ledoux1, Catherine S Woolley

  • 1Department of Neurobiology and Physiology and Northwestern University Institute for Neuroscience, Northwestern University, Evanston, Illinois 60208, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|January 28, 2005
PubMed
Summary

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Correction: Acute inhibition of neurosteroid estrogen synthesis suppresses status epilepticus in an animal model.

eLife·2016

Estrogen reduces available synaptic vesicles in inhibitory synapses, impacting GABA release and neuronal function. This study reveals a new mechanism for estrogen

Area of Science:

  • Neuroscience
  • Cell Biology
  • Endocrinology

Background:

  • Estrogen influences neuronal excitability, particularly in the hippocampus.
  • Disinhibition of hippocampal CA1 pyramidal cells is a known effect of estrogen.
  • Mechanisms underlying estrogen's effects on inhibitory synapses are not fully understood.

Purpose of the Study:

  • To investigate structural changes in inhibitory synapses of hippocampal CA1 pyramidal cells after estrogen treatment.
  • To determine if estrogen alters the number of inhibitory inputs or modifies inhibitory boutons.
  • To elucidate the role of synaptic vesicle availability in estrogen-mediated synaptic inhibition.

Main Methods:

  • Three-dimensional reconstruction from serial electron micrographs.
  • Analysis of inhibitory boutons and synapses in estrogen-treated and control animals.

Related Experiment Videos

  • Quantification of synaptic vesicles adjacent to the presynaptic membrane.

    • Main Results:

    • Estrogen specifically decreases the number of synaptic vesicles near the presynaptic membrane of inhibitory synapses.
    • No significant difference was found in the overall number of vesicles per bouton.
    • The density of inhibitory inputs per neuron remained unchanged.

    Conclusions:

    • Estrogen modulates synaptic inhibition by reducing the pool of readily releasable GABA vesicles.
    • This study provides the first in vivo evidence that presynaptic vesicle availability is a regulated synaptic property.
    • Findings offer a novel mechanism for estrogen's functional effects on hippocampal synaptic physiology.