Related Experiment Video
Updated: Aug 19, 2026

Identification of Potential Anti-TB Candidates: A Step-by-Step Guide to Synthesis, MIC Determination, and Cytotoxicity Assessment in Mammalian Cells
Published on: May 22, 2026
NMR spectroscopic analysis of new spiro-piperidylrifamycins
Eduardo Rubio1, Isabel Merino, Ana-Belén García
1Instituto Universitario de Química Organometálica Enrique Moles, Unidad Asociada al CSIC, Universidad de Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain. erubio@uniovi.es
Abstract:
New spiro-piperidylrifamycin derivatives are presented. These compounds were synthesized from the reaction of 3-amino-4-iminorifamycin S and enantiomerically pure 4-piperidones, which generate diasteroisomeric rifabutin analogues with a new stereocentre at the spiranic carbon. The (1)H and (13)C NMR spectra of these new compounds, and also the configuration of the new stereogenic centres, were assigned using 2D NMR spectroscopic techniques. A preliminary study of the (1)H and (13)C NMR spectra of the starting compounds rifamycin S, 3-amino-4-iminorifamycin S and the related rifabutin was also carried out and as a result, their previously published (13)C NMR data were corrected.
Related Concept Videos
NMR Spectroscopy of Aromatic Compounds
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
Spectroscopy of Carboxylic Acid Derivatives
In the...
¹H NMR: Complex Splitting
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
NMR Spectroscopy Of Amines

