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Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Published on: July 12, 2018
Biodiversity analysis of microbial community in the chem-bioflocculation treatment process
Siqing Xia1, Feng Wang, Yigang Fu
1State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Engineering, Tongji University, 200092 Shanghai, China. siqingxia@mail.tongji.edu.cn
Abstract:
Total DNA was directly extracted from environmental samples and amplified with polymerase chain reaction (PCR) technique. The PCR products were fingerprinted via denaturing gradient gel electrophoresis (DGGE). Significant differences were observed in the microbial community structures between traditional treatment process and chem-bioflocculation process. The microbial community structure shift at different sampling locations in chem-bioflocculation process and on two typical operational conditions was studied. 16S rDNA V3 regions of some dominant species were sequenced and the species were identified. The microbial communities were stable in both the chem-bioflocculation process and the activated sludge process under various experimental conditions presented in this work. The attached growth treatment process was less stable when operational conditions changed.
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