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Novel approach to quantify immobilized-enzyme distributions

J L van Roon1, E Groenendijk, H Kieft

  • 1Department of Agrotechnology and Food Sciences, Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. jeroen.vanroon@wur.nl

Summary

This study quantifies enzyme distribution in immobilized penicillin-G acylase (Assemblase) particles. Results reveal heterogeneous enzyme loading, concentrated in outer particle regions, informing biocatalyst design.

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