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Updated: Feb 18, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Rapid separation of microorganisms by quartz microchip capillary electrophoresis
Tomoyoshi Shintani1, Masaki Torimura, Hiroaki Sato
1Textile Industrial Institute of Ehime Prefecture, 2-5-48 Higashimura-minami Imabari, Ehime 799-1507, Japan. shintani@iri.pref.ehime.jp
Abstract:
We developed and optimized a system coupling microchip capillary electrophoresis (MCE) and laser-induced fluorescence (LIF) detection for the analysis of microorganisms. The MCE-LIF system successfully separated pure cultures of lactic acid bacteria and Saccharomyces cerevisiae within 200 s. The results indicate that the MCE system can be conveniently used for the rapid and highly sensitive detection of microorganisms. Thus, MCE can provide a cheap and simple method for the on-line detection of microbial contamination.
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