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Estimation of circulating blood volume in infants using the pulse dye densitometry method

Keiko Nagano1, Takashi Kusaka, Kensuke Okubo

  • 1Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan.

Paediatric Anaesthesia
|January 29, 2005
PubMed

Insights

Pulse dye densitometry (PDD) using indocyanine green (ICG) accurately estimates hemodynamics in infants. This noninvasive method allows for bedside monitoring of circulating blood volume in neonates and critically ill infants.

Area of Science:

  • Neonatal physiology
  • Pediatric critical care
  • Hemodynamic monitoring

Background:

  • Accurate hemodynamic assessment is crucial for critically ill infants.
  • Pulse dye densitometry (PDD) with indocyanine green (ICG) is a novel bedside method for measuring circulating blood volume, previously established in adults.

Purpose of the Study:

  • To evaluate the applicability and accuracy of PDD using ICG for hemodynamic monitoring in infants.
  • To determine if PDD can reliably measure circulating blood volume in a neonatal population.

Main Methods:

  • A validation study compared arterial indocyanine green concentrations measured by blood sampling (spectrophotometry) against noninvasive PDD measurements in seven infants.
  • Circulating blood volume was subsequently estimated using the PDD method in 25 infants (gestational age 24-40 weeks).

Main Results:

  • A strong positive correlation (r = 0.913, P < 0.0001) was observed between ICG concentrations measured by blood sampling and PDD.
  • Bland Altman analysis showed minimal bias (-0.24 +/- 0.30 mg.l(-1)) and acceptable limits of agreement between the two methods.
  • The mean (SD) blood volume measured by PDD was 94.9 (24.3) ml.kg(-1), consistent with previously reported values.

Conclusions:

  • Pulse dye densitometry (PDD) utilizing indocyanine green (ICG) is a validated and accurate method for hemodynamic monitoring in infants.
  • This noninvasive technique offers a reliable approach for bedside assessment of circulating blood volume in the neonatal population.
Abstract

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