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A protocol for direct sequencing of multiple gene specific PCR products from Discula umbrinella, a fungal endophyte,
1Policy and Program Development, U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Riverdale, MD, 20737, USA. susan.d.cohen@aphis.usda.gov
Journal of Microbiological Methods
|January 29, 2005
Abstract:
A protocol for direct sequencing of polymerase chain reaction (PCR) products from mycelia of Discula umbrinella, a fungal endophyte, using bufferless electrophoresis is described. This improved method allows researchers to conduct high-capacity screening of multiple gene regions for fungal endophytes applicable to microbial ecology and population genetic studies.
