Related Experiment Video

Updated: Aug 19, 2026

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography
06:44

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

Published on: April 13, 2018

Polymorphonuclear leukocyte integrins in deep venous thrombosis

G Caimi1, B Canino, F Ferrara

  • 1Department of Internal Medicine, Cardiovascular and Renal Diseases, Università di Palermo, Palermo, Italy.

Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
|January 29, 2005
PubMed

Insights

Polymorphonuclear leukocytes (PMN) in deep venous thrombosis (DVT) show altered CD11b and CD11c integrin expression. DVT patients exhibit impaired PMN activation responses, suggesting therapeutic targets for preventing vein damage.

Area of Science:

  • Hematology
  • Immunology
  • Vascular Biology

Background:

  • Polymorphonuclear leukocytes (PMN) play a role in deep venous thrombosis (DVT) pathophysiology.
  • Beta(2)-integrins on PMNs are crucial for leukocyte adhesion and migration.

Purpose of the Study:

  • To investigate the phenotypical expression of PMN beta(2)-integrins (CD11a, CD11b, CD11c) in individuals with leg DVT.
  • To compare integrin expression and activation responses in DVT patients versus healthy controls.

Main Methods:

  • Flow cytofluorimetry was used to analyze PMN beta(2)-integrin expression (CD11a, CD11b, CD11c) in 19 DVT subjects.
  • PMN cells were evaluated at baseline and after in vitro activation with PMA and fMLP.

Main Results:

  • DVT subjects showed decreased CD11b and increased CD11c expression at baseline compared to controls.
  • In normal subjects, PMA and fMLP increased all PMN adhesion molecules.
  • In DVT subjects, CD11a expression remained unchanged after activation, unlike in controls.

Conclusions:

  • Altered PMN integrin expression and impaired activation in DVT patients may contribute to disease pathophysiology.
  • These findings suggest potential therapeutic strategies targeting PMN integrins to prevent post-thrombotic vein dysfunction.

More Related Videos

Stenosis of the Inferior Vena Cava: A Murine Model of Deep Vein Thrombosis05:37

Stenosis of the Inferior Vena Cava: A Murine Model of Deep Vein Thrombosis

Published on: December 22, 2017

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice05:12

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Published on: August 13, 2015

Related Concept Videos

Venous Thrombosis I: Introduction01:30

Venous Thrombosis I: Introduction

Venous thrombosis, the most common disorder of the veins, involves the formation of a thrombus or blood clot associated with vein inflammation. It can be classified as either superficial vein thrombosis or deep vein thrombosis.Superficial Vein Thrombosis: This involves the formation of a thrombus in a superficial vein, usually the greater or lesser saphenous vein. Though less severe than deep vein thrombosis (DVT), SVT can lead to complications if untreated.Deep Vein Thrombosis (DVT): This...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies01:20

Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies

The key difference between Superficial Vein Thrombosis (SVT) and Deep Vein Thrombosis (DVT) lies in their location and severity.Clinical ManifestationsSVT typically presents with localized pain, tenderness, and redness along the course of a superficial vein, often accompanied by a palpable, cord-like structure under the skin. This condition is usually less dangerous than DVT but can be uncomfortable and may lead to complications such as cellulitis or, rarely, a clot extension into the deep...
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.