Low temperature-induced fatty acid desaturation in Brassica napus: thermal lability of the process

The leaves of Brassica napus plants grown at 5 degrees C have a higher rate of fatty acid desaturation in both the cytosolic and chloroplastic pathways of diacylglycerol biosynthesis than plants grown at higher (up to 30 degrees C) temperatures. This physiological response to low growth temperature results in higher levels of unsaturated fatty acids in the leaf membrane lipids. These data suggest that this low temperature-induced desaturation process is thermolabile and can be inactivated by placing the leaves at temperatures of 30 degrees C for 4-8 h. Our evidence suggests that it is an additional rapid process to the normal 'basal' desaturation which occurs at a relatively slower rate. The data also show that the C16 and C18 fatty acids in the cytosol are desaturated at different rates and react differently to the high temperature treatment, suggesting that they are separate processes and are controlled independently.