Related Experiment Video
Updated: Aug 19, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
An unambiguous assignment method by 2D selective-TOCSY-HSQC and selective-TOCSY-DQFCOSY and structural analysis by
Hajime Sato1, Yasuhiro Kajihara
1Bruker Biospin K.K., 3-21-5 Ninomiya, Tsukuba, Ibaraki 305-0051, Japan. hajime.sato@bruker-biospin.jp
Abstract:
In this paper we present a newly developed 2D selective-TOCSY-HSQC experiment for unambiguous assignment of individual sugar components of a biantennary undecasaccharide. The correlation signals between the protons and carbons of a selectively excited sugar component in the undecasaccharide were observed in a 2D selective-TOCSY-HSQC experiment. The network of proton signals of this sugar was observed in a 2D selective-TOCSY-DQFCOSY experiment. These two experiments enabled us to perform unambiguous sequential assignment of both protons and carbons of the individual sugar components in the undecasaccharide. In addition, a three-dimensional structural analysis was performed by a 2D selective-TOCSY-NOESY experiment, which clearly distinguishes between inter-sugar and intra-sugar ring NOE signals.
Related Concept Videos
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

