Transcriptional regulation and the role of diverse coactivators in animal cells
Related Experiment Video
Updated: Aug 9, 2026

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
Published on: November 6, 2017
1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. roeder@rockfeller.edu
Abstract:
Transcriptional regulation in eukaryotes involves structurally and functionally distinct nuclear RNA polymerases, corresponding general initiation factors, gene-specific (DNA-binding) regulatory factors, and a variety of coregulatory factors that act either through chromatin modifications (e.g. histone acetyltransferases and methyltransferases) or more directly (e.g. Mediator) to facilitate formation and function of the preinitiation complex. Biochemical studies with purified factors and DNA versus recombinant chromatin templates have provided insights into the nature and mechanism of action of these factors, including pathways for their sequential function in chromatin remodeling and preinitiation complex formation/function (transcription) steps and a possible role in facilitating the transition between these steps.
Related Concept Videos
RNA Polymerase II Accessory Proteins
Co-activators and Co-repressors
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
General Transcription Factors
RNA Polymerase II Accessory Proteins
Co-activators and Co-repressors

