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Comparison of intraperitoneal anti-adhesive polysaccharides derived from Phellinus mushrooms in a rat peritonitis
Jae-Sung Bae1, Kwang-Ho Jang, Hee-Kyung Jin
1Department of Surgery, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Aim:
To assess the adhesion- and abscess-reducing capacities of various concentrations of polysaccharides derived from fungus, Phellinus gilvus (PG) or Phellinus linteus (PL) in a rat peritonitis model.
Methods:
In 96 SD rats, experimental peritonitis was induced using the cecal ligation and puncture model (CLP). Rats were randomly assigned to 8 groups; Ringer's lactate solution (RL group), hyaluronic acid (HA group), 0.025%, 0.25%, and 0.5% polysaccharides from PG (PG0.025, 0.25, and 0.5 groups), and PL (PL0.025, 0.25, and 0.5 groups). Adhesions and abscesses were noted at 7 d after CLP. RT-PCR assay was performed to assess the cecal tissue.
Results:
Adhesion formation was significantly reduced in PG0.25, 0.5, PL0.25, 0.5, and HA groups (2.5+/-0.7, 2.4+/-0.7, 3.8+/-1.0, 3.6+/-0.8, and 2.7+/-1.1, P<0.05). The incidence of abscesses was significantly reduced in all treated groups compared to RL group (58%, P<0.05). The urokinase-type plasminogen activator (uPA) gene expression was greatly up-regulated by increasing the concentration of polysaccharides. The urokinase-type plasminogen activator receptor (uPAR) and tumor necrosis factor (TNF)-alpha mRNA were highly expressed in PG0.25, 0.5, PL0.25, and 0.5 groups.
Conclusion:
We concluded that 0.5% polysaccharide derived from PG and PL was the optimal concentration in preventing adhesion and abscess formation and may act by modulating activity of uPA and TNF-alpha in a rat peritonitis model.
Insights
Polysaccharides from Phellinus gilvus and Phellinus linteus fungi significantly reduced adhesions and abscesses in a rat peritonitis model. The optimal concentration of 0.5% may modulate urokinase-type plasminogen activator and tumor necrosis factor-alpha activity.
Area of Science:
- Biochemistry
- Immunology
- Pharmacology
Background:
- Peritonitis is a serious intra-abdominal infection.
- Adhesions and abscesses are common complications of peritonitis.
- Fungal polysaccharides are known for their immunomodulatory properties.
Purpose of the Study:
- To evaluate the efficacy of Phellinus gilvus (PG) and Phellinus linteus (PL) polysaccharides in reducing adhesions and abscesses.
- To determine the optimal concentration of these fungal polysaccharides for therapeutic use.
- To investigate the molecular mechanisms underlying their anti-inflammatory effects.
Main Methods:
- Experimental peritonitis was induced in rats using the cecal ligation and puncture (CLP) model.
- Rats were treated with varying concentrations of PG or PL polysaccharides, or hyaluronic acid.
- Adhesion and abscess formation were assessed, and cecal tissue was analyzed using RT-PCR.
Main Results:
- PG and PL polysaccharides significantly reduced adhesion formation at concentrations of 0.25% and 0.5%.
- All treated groups showed a significant reduction in abscess incidence compared to the control group.
- Polysaccharide treatment upregulated urokinase-type plasminogen activator (uPA) and increased expression of uPA receptor (uPAR) and tumor necrosis factor-alpha (TNF-alpha) mRNA.
Conclusions:
- 0.5% PG and PL polysaccharides are optimal for preventing adhesions and abscesses in a rat peritonitis model.
- These fungal polysaccharides may exert their effects by modulating uPA and TNF-alpha activity.
- Fungal polysaccharides show potential as therapeutic agents for peritonitis complications.
