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clc is co-expressed with clf or cntfr in developing mouse muscles
Béatrice de Bovis1, Damien Derouet, Jean-François Gauchat
1INSERM UMR 623, Developmental Biology Institute of Marseille (CNRS – INSERM – Univ, Méditerranée), Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France. delapeyr@ibdm.univ-mrs.fr.
Cell Communication and Signaling : CCS
|February 3, 2005
Summary
Cardiotrophin-like cytokine (CLC) and its partners CLF or sCNTFR are crucial for motor neuron development. Their expression patterns support their role in signaling via the ciliary neurotrophic factor (CNTF) receptor.
Area of Science:
- Neuroscience
- Molecular Biology
- Developmental Biology
Background:
- The ciliary neurotrophic factor (CNTF) receptor complex, comprising gp130, leukemia inhibitory factor receptor, and CNTF receptor alpha (CNTFR), is vital for motor neuron development.
- The precise ligands activating this receptor complex during development remain incompletely understood.
- Two novel heterodimeric ligands, cardiotrophin-like cytokine (CLC) with either cytokine-like factor-1 (CLF) or soluble CNTFR (sCNTFR), have been recently identified.
Purpose of the Study:
- To investigate the expression patterns of CLC, CLF, and sCNTFR during mouse development.
- To determine if CLC, CLF, and sCNTFR are co-expressed, suggesting a functional relationship.
- To evaluate the potential role of CLC/CLF or CLC/sCNTFR complexes in motor neuron development mediated by the CNTF receptor.
Main Methods:
- Analysis of CLC expression during embryonic and postnatal development in mice.
- Examination of CLC co-expression with CLF and CNTFR at the single-cell level.
- Correlation of expression patterns with known periods of motor neuron development and loss.
Main Results:
- CLC expression was detected in tissues critical for development, including lung, kidney, vibrissae, tooth, epithelia, and muscles.
- This expression coincided with developmental periods where motor neuron deficits are observed in mice lacking a functional CNTF receptor.
- CLC was found to be co-expressed with CLF and CNTFR at the single-cell level, indicating potential co-secretion.
Conclusions:
- The expression and co-localization patterns strongly suggest that CLC, in association with either CLF or sCNTFR, acts as a key ligand for the CNTF receptor.
- These findings support the hypothesis that CLC-containing complexes are essential for the signaling required for proper motor neuron development.
- This study elucidates the role of novel CLC-based ligands in CNTF receptor-mediated developmental processes.