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The transillumination possibility of imidazole-osmium postfixed tissue and its consequences for the handling of

Tilman Voigt1, Wolfgang Dauber

  • 1Institute of Anatomy, University of Fribourg, Rte. Albert-Gockel 1, CH-1700 Fribourg, Switzerland. tilman.voigt-gawatz@unifr.ch

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|February 3, 2005
PubMed

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...