Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An alternative sampling algorithm for use in liquid chromatography/tandem mass spectrometry experiments.

Bernhard M Kohli1, Jimmy K Eng, Roger M Nitsch

  • 1Division of Psychiatry Research, August Forel-Strasse 1, 8008 Zürich, Switzerland. bernhard.kohli@bli.unizh.ch

Rapid Communications in Mass Spectrometry : RCM
|February 3, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cliramitug for depletion of cardiac amyloid transthyretin: long-term follow-up of the NI006-101 trial.

Nature medicine·2026
Same author

Site Confidence Scores among All Available Structures to Help Infer Conformational Origins of Identified Cross-Links.

Journal of proteome research·2026
Same author

Independent and interactive contributions of white matter hyperintensities and Alzheimer's disease imaging and plasma biomarkers to cognitive decline in older adults without dementia.

Alzheimer's research & therapy·2026
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Increased levels of GFAP and purinergic P2X7 receptor in Alzheimer's disease brain are associated with Aβ, tau pathologies and synaptic loss.

Alzheimer's research & therapy·2025
Same author

Predicting cognitive decline with amyloid-PET, plasma p-tau217, Aβ42/40, and p-tau217/Aβ42 in a community-based cohort - relevance for clinical trial enrollment.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

A new algorithm, Fulspec, improves liquid chromatography/tandem mass spectrometry (LC/MS/MS) by dynamically excluding peptide precursors based on chromatographic properties, enhancing data quality and analytical efficiency.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Liquid chromatography/tandem mass spectrometry (LC/MS/MS) is crucial for analyzing complex peptide mixtures.
  • Dynamic exclusion functions are employed in LC/MS/MS to prevent repeated selection of identical precursors for fragmentation.
  • Current methods often rely on rigid time windows for exclusion, which can be suboptimal.

Purpose of the Study:

  • To introduce and evaluate a novel algorithm for dynamic exclusion of m/z values in LC/MS/MS.
  • To improve the efficiency and quality of peptide identification by optimizing precursor selection.
  • To challenge the assumption that high precursor abundance guarantees high-quality identifications.

Main Methods:

  • Development of the Full-scan based peak exclusion (Fulspec) algorithm.

Related Experiment Videos

  • Fulspec utilizes a simplified model of chromatographic peak formation to detect and exclude contaminants.
  • The algorithm dynamically excludes m/z values based on chromatographic properties rather than fixed time windows.

    • Main Results:

    • Fulspec achieved a distribution of collision-induced dissociation (CID) spectra with minimal retention time axis tailing.
    • The algorithm effectively excluded low-quality CID spectra by considering intensity biases.
    • This approach optimizes the use of analytical capacity by freeing it from redundant or low-value selections.

    Conclusions:

    • Fulspec offers an improved method for dynamic exclusion in LC/MS/MS analyses.
    • The algorithm enhances data quality and analytical efficiency by leveraging chromatographic information.
    • Further validation and integration by instrument manufacturers are recommended for broader adoption.