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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Published on: November 4, 2021
Database independent detection of isotopically labeled MS/MS spectrum peptide pairs
Frank Potthast1, Jiri Ocenasek, Dorothea Rutishauser
1Functional Genomics Center Zurich, Uni/ETH Zurich, Winterthurer Strasse 190, 8057 Zurich, Switzerland. frank@fgcz.ethz.ch
Abstract:
Mass spectrometry data generated in differential profiling of complex protein samples are classically exploited using database searches. In addition, quantitative profiling is performed by various methods, one of them using isotopically coded affinity tags, where one typically uses a light and a heavy tag. Here, we present a new algorithm, ICATcher, which detects pairs of light/heavy peptide MS/MS spectra independent of sequence databases. The method can be used for de novo sequencing and detection of posttranslational modifications. ICATcher is distributed as open source software.
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