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Bioengineering Human Microvascular Networks in Immunodeficient Mice
06:55

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Published on: July 11, 2011

Tissue-engineered human vascular media with a functional endothelin system.

Karina Laflamme1, Charles J Roberge, Julie Labonté

  • 1Laboratoire d'Organogénèse Expérimentale/LOEX, Hôpital Saint-Sacrement du CHA, and Department of Surgery, Laval University, Québec, PQ, Canada.

Circulation
|February 3, 2005
PubMed
Summary

Tissue-engineered blood vessels utilizing human cells show functional endothelin ET(A) receptors and endothelin-converting enzyme. This suggests potential for controlling vascular resistance in regenerative medicine applications.

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Area of Science:

  • Regenerative Medicine
  • Vascular Biology
  • Biomaterials Science

Background:

  • Cardiovascular diseases are a leading cause of mortality globally.
  • Current treatments include surgical procedures and transplantation, with limitations.
  • There is a need for patient-specific vascular grafts using human components.

Purpose of the Study:

  • To investigate the functionality of the endothelinergic system in a novel tissue-engineered human blood vessel.
  • To assess the presence and activity of endothelin receptors and related enzymes.

Main Methods:

  • Tissue-engineered human blood vessels were constructed using a self-assembly method with human cells.
  • Vasoconstriction studies were performed using selective agonists and antagonists for endothelin receptors.
  • Reverse-transcriptase polymerase chain reaction (RT-PCR) was used to detect receptor mRNA.
  • Endothelin-converting enzyme activity was assessed.

Main Results:

  • Functional endothelin ET(A) receptors were identified in the engineered vascular media.
  • RT-PCR confirmed the presence of ET(A) receptor mRNA, but not ET(B) receptor mRNA.
  • The endothelin-converting enzyme was found to be present and functional in the engineered vessels.

Conclusions:

  • The media of the tissue-engineered blood vessel possesses functional endothelin ET(A) receptors.
  • The presence of endothelin-converting enzyme further supports the vessel's potential for vascular tone regulation.
  • These findings indicate the engineered vessel's capacity to control vascular resistance.