Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Host gene expression changes and DNA amplification during temperate phage induction.

Jonathan G Frye1, Steffen Porwollik, Felisa Blackmer

  • 1Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.

Journal of Bacteriology
|February 3, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A screen of Salmonella enterica mutants interacting with fresh onions and alfalfa sprouts.

International journal of food microbiology·2026
Same author

The quality and safety of Rhodiola rosea supplements on the U.S. market: An analysis of biomarkers, heavy metals, and pesticide residues.

PloS one·2026
Same author

YeiE regulates YeiH to implement sulfite stress resistance in <i>Salmonella enterica</i> serotype Typhimurium.

Journal of bacteriology·2026
Same author

Molybdenum-independent [4Fe-4S]-Catalyzed Sulfate Assimilation Sustains <i>Salmonella</i> Virulence.

Research square·2025
Same author

Amino acid decarboxylation preserves <i>Salmonella</i> fitness during phagocyte-derived oxidative stress.

mBio·2025
Same author

Comparison of Mineral Levels in Blood and Hair Samples of Healthy Adults: Evaluating the Clinical Utility of Hair Mineral Analysis.

Biological trace element research·2025

Salmonella Typhimurium prophages can trigger host genome amplification, termed escape replication, during lytic induction. This phenomenon, observed with Fels-1 and Gifsy-1 phages, suggests widespread host genome instability during phage replication.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Salmonella enterica serovar Typhimurium LT2 harbors four temperate prophages.
  • Prophage induction initiates a lytic cycle, impacting host cell processes.

Purpose of the Study:

  • To investigate phage-host genome interactions during lytic induction.
  • To characterize the phenomenon of escape replication in Salmonella prophages.

Main Methods:

  • Induction of lytic cycle using hydrogen peroxide or mitomycin C.
  • Microarray analysis to monitor phage genome copy number and host RNA expression.
  • Genetic manipulation of prophage genes (xis, integrase).

Main Results:

  • Phage gene transcription was temporally classified, and host genes responding to induction were identified.

Related Experiment Videos

  • Fels-1 prophage induced significant host genome amplification (escape replication) prior to excision.
  • Mutations in Fels-1 excisionase (xis) or Gifsy-1 integrase/excisionase genes also triggered escape replication.
  • Host genome amplification was observed in other Salmonella Typhimurium strains (SL1344, 14028s) upon phage induction.

    • Conclusions:

    • Escape replication, a host genome amplification phenomenon, is induced by Salmonella prophages during lytic cycles.
    • This process is linked to phage replication mechanisms and can be modulated by specific phage genes.
    • Escape replication may be a widespread event in Salmonella and other bacterial species harboring prophages.