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Related Experiment Videos

Mapping segmental and sequence variations among laboratory mice using BAC array CGH.

Antoine M Snijders1, Norma J Nowak, Bing Huey

  • 1Cancer Research Institute, University of California San Francisco, San Francisco, California 94143, USA.

Genome Research
|February 3, 2005
PubMed
Summary
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This study maps copy number variation in mouse strains using BAC arrays. It identifies 74 variant loci that differentiate laboratory mouse strains and offers a new genotyping method.

Area of Science:

  • Genomics
  • Comparative genomics
  • Mouse genetics

Background:

  • Understanding sequence variation is crucial for interpreting mouse models in research.
  • Copy number variation (CNV) represents a significant source of genetic diversity.
  • Differentiating between mouse strains requires detailed genetic mapping.

Purpose of the Study:

  • To map sequence variation, specifically copy number variation, across different laboratory mouse strains.
  • To identify genetic markers that distinguish between Mus spretus and Mus musculus strains.
  • To develop an efficient method for genotyping interspecific backcross mice.

Main Methods:

  • Utilized arrays of 2069 bacterial artificial chromosomes (BACs) for comparative mapping.
  • Analyzed sequence variation and copy number changes using log(2) ratio.

Related Experiment Videos

  • Employed fluorescence in situ hybridization (FISH) for chromosomal mapping.

    • Main Results:

    • Identified 80 BAC clones representing 74 autosomal loci with copy number variation.
    • These variant loci effectively distinguish between laboratory mouse strains.
    • FISH mapping confirmed single-site localization for 63 BACs and multi-site for 17.
    • Demonstrated that subtle ratio changes can distinguish homozygous and heterozygous genomic regions.

    Conclusions:

    • BAC arrays are effective for mapping copy number variation in mice.
    • Identified specific loci that differentiate laboratory mouse strains.
    • Developed a sensitive method for genotyping using copy number variation analysis in backcross progeny.