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In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
Published on: August 20, 2019
Different P105 promoter activities among natural variants of human papillomavirus type 18
Laura Sichero1, Eduardo Luis Franco, Luisa Lina Villa
1Instituto de Química, Universidade de São Paulo, Cidade Universitária, and Department of Virology, Ludwig Institute for Cancer Research, São Paulo, Brazil.
Abstract:
Association between non-European variants of human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 and cervical lesions has been suggested. To compare the P105 promoter activity among 6 HPV-18 variants, their complete long control regions (LCRs), as well as that of HeLa cells, were cloned upstream of the luciferase gene and transiently transfected in C33 cells. Whereas the B18-2 European variant showed the lowest promoter activity, the Asian-Amerindian variant, B18-3, had the highest activity. All variants tested were more active than the P97 HPV-16 prototype promoter. Differences in the LCR that impact P105 promoter activity could be responsible for the observed variability in oncogenic potential.
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