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Updated: Aug 19, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Optical saturation in fluorescence correlation spectroscopy under continuous-wave and pulsed excitation
Ingo Gregor1, Digambara Patra, Jörg Enderlein
1Institute for Biological Information Processing 1, Forschungszentrum Jülich, 52425 Jülich, Germany.
Abstract:
A detailed theoretical and experimental study of the dependence of fluorescence correlation measurements on optical excitation power due to optical saturation effects is presented. It is shown that the sensitivity of a fluorescence correlation measurement on excitation power becomes increasingly stronger for decreasing excitation power. This makes exact measurements or diffusion coefficients with fluorescence correlation spectroscopy rather difficult. A strong difference of this behavior for continuous-wave and pulsed excitation is found.
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