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[Analysis of nine autoantibodies associated with systemic autoimmune diseases using the Luminex technology. Results
A Buliard1, F Fortenfant, P Ghillani-Dalbin
1Laboratoire d'immunologie, CHU, Hôpital du Bocage, Dijon.
Unlabelled:
Luminex technology allows simultaneous detection of several analytes in a single well. Applications have been recently developed for the detection of autoantibodies.
Purpose:
To evaluate the performances and convenience of the Fidis analytical system (BioMedical Diagnostics, Marnes-la-Vallee, France) for the detection of nine autoantibodies associated with connective diseases: SS-A, SS-B, Sm, RNP, Scl-70, Jo-1, CENP-B, P ribosomal and double stranded DNA antibodies.
Materials And Methods:
Three hospital laboratories analysed 366 samples taken from their serum banks and corresponding to the main systemic autoimmune diseases. Control samples included 120 sera from blood donors and 42 sera from patients with dysglobulinemia.
Results:
In each laboratory, handling of this new analytical system was easy. Results are readily obtained: nine autoantibodies are quantitated in fourty-four sera in less than two hours. A clear-cut discrimination between negative and positive results was observed, due to very low backgrounds. Intra-assay and inter-assay variations were low: coefficients of variation were under 10% in 80 and 64% of the cases, respectively. Results obtained with Fidis correlated satisfactorily with those obtained with the numerous routine techniques used in each laboratory. The overall concordance exceeded 93%.
Conclusion:
Fidis is a reliable and time-saving analytical system for the detection of autoantibodies associated with systemic autoimmune diseases.
