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Related Experiment Videos

Microbial adherence on poly(methyl methacrylate) (PMMA) surfaces.

C C Chang1, K Merritt

  • 1Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106.

Journal of Biomedical Materials Research
|February 1, 1992
PubMed
Summary
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Bacterial adherence to biomaterials like poly(methyl methacrylate) (PMMA) is a key factor in implant infections. This study quantifies bacterial adhesion to PMMA, finding that gentamicin-treated samples reduce viable bacteria but can still form dead biofilms.

Area of Science:

  • Biomaterials Science
  • Infectious Diseases
  • Microbiology

Background:

  • Implanted biomaterials are susceptible to infection, often by polymicrobial or low-virulence organisms.
  • Understanding bacterial adherence to biomaterials is crucial for preventing implant-associated infections.

Purpose of the Study:

  • To quantify bacterial adherence to poly(methyl methacrylate) (PMMA) using two distinct methods.
  • To investigate the effect of gentamicin on bacterial adherence and viability on PMMA.
  • To analyze the adherence patterns of Staphylococcus epidermidis and Proteus mirabilis, individually and in combination.

Main Methods:

  • Bacteria were eluted from PMMA samples using ultrasonic cleaning.
  • Quantification of adhered bacteria was performed using colony counts (viable organisms) and particle counting (viable and nonviable organisms).

Related Experiment Videos

  • Experiments involved Staphylococcus epidermidis, Proteus mirabilis, and PMMA with and without gentamicin.

    • Main Results:

    • Staphylococcus epidermidis generally showed higher adherence than Proteus mirabilis.
    • Co-culture of bacteria altered adherence patterns, increasing Proteus mirabilis adherence and affecting Staphylococcus epidermidis adherence.
    • PMMA with gentamicin showed adherence of nonviable organisms, with reduced adherence over time compared to PMMA alone.
    • A significant dead biofilm mass was observed on PMMA with gentamicin.

    Conclusions:

    • Both viable and nonviable bacterial quantification methods provide valuable insights into adherence to PMMA.
    • Gentamicin-treated PMMA reduces viable bacterial adherence but can lead to dead biofilm formation.
    • Dead biofilms on gentamicin-treated PMMA may serve as a nidus for subsequent bacterial colonization and late infections.