Related Experiment Video
Updated: Aug 19, 2026
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
An enzyme-amplified diffraction-based immunoassay
Richard W Loo1, Pui L Tam, Jane Betty Goh
1Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Canada M5S 3H6. rloo@chem.utoronto.ca
Abstract:
We have previously shown that a gold-conjugated secondary label can be used to reduce the limit of detection in a diffraction-based assay by more than 40-fold. We now show that by using a combination of a peroxidase-conjugated secondary label and a precipitating substrate the limit of detection in a diffraction-based assay can be reduced by more than 1000-fold. The response to secondary enhancement was linear for concentrations from 50 to 2000 pg/mL of antidigoxin.
