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Updated: Aug 19, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Pathophysiological functions of nitric oxide-mediated protein modifications
Harry Ischiropoulos1, Andrew Gow
1Stokes Research Institute, Children's Hospital of Philadelphia, University of Pennsylvania, Abramson Research Center, Rm. 416, 34th Street, Civic Center Building, Philadelphia, PA 19104, USA. ischirop@mail.med.upenn.edu
Abstract:
Specific post-translational modifications resulting from the reaction of nitric oxide or nitric oxide-derived reactive nitrogen species with selective proteins may explain the pleiotropic effects of this molecule in biological systems. Three specific reactions, binding to metal centers, nitrosation of nucleophilic centers and nitration of aromatic residues constitute a major component of the biological reactivity of nitric oxide. We postulate that the chemical reactivity of nitric oxide within biological systems allows this simple diatomic molecule to operate as an integrator of physiological homeostasis and potentially under not completely understood circumstances as a mediator of pathological phenotypes. This chapter reviews the chemistry, putative biological functions and significance of the principal nitric oxide-mediated proteins modifications.
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