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Identifying Protein-protein Interaction Sites Using Peptide Arrays
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Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein

Hyeon-Su Ro1, Sun Ok Jung, Byung Hoon Kho

  • 1BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea.

Applied and Environmental Microbiology
|February 5, 2005
PubMed

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...