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Published on: October 23, 2018
Histochemical localization of acid mannose 6-phosphatase activity in the newborn rat hepatocytes
S A Kalamidas1, O B Kotoulas, A C Hann
1Department of Anatomy, Histology and Embryology, Medical School, University of Ioannina, Ioannina 451 10, Greece. skalamid@cc.uoi.gr
Abstract:
The localization of acid mannose 6-phosphatase activity in newborn rat hepatocytes was demonstrated at the electron microscopic level by using a histochemical method based on the work of Robinson and Karnovsky. Reaction product was virtually restricted to the lysosomes. Most of them exhibited various grades of reactivity. Some were devoid of activity. Our observations suggested that this histochemical method could be used to differentiate distinct subpopulations of lysosomes on the basis of their acid mannose 6-phosphatase activity.
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