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Related Experiment Videos

AC133/CD133/Prominin-1.

Sergey V Shmelkov1, Ryan St Clair, David Lyden

  • 1Weill Medical College of Cornell University, Department of Genetic Medicine, 1300 York Avenue, New York, NY 10021, USA. svs2001@med.cornell.edu

The International Journal of Biochemistry & Cell Biology
|February 8, 2005
PubMed
Summary
This summary is machine-generated.

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Prominin-1 (CD133) is a cell-surface glycoprotein found on various stem cells. While its exact function remains unclear, the AC133 epitope is a valuable marker for isolating hematopoietic and endothelial progenitor cells.

Area of Science:

  • Cell Biology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Prominin-1 is a cell-surface glycoprotein with five transmembrane domains, originally identified on mouse neuroepithelial stem cells.
  • Human Prominin-1 (CD133) was isolated from hematopoietic stem cells using the AC133 epitope.
  • Prominin-1 expression is regulated by multiple tissue-specific promoters, leading to diverse mRNA isoforms.

Purpose of the Study:

  • To investigate the role of Prominin-1 in key stem cell functional features.
  • To understand the biological function of Prominin-1 in different stem cell types.
  • To highlight the utility of the AC133 epitope as a marker.

Main Methods:

  • Analysis of Prominin-1 expression patterns across various stem cell populations.

Related Experiment Videos

  • Investigating the functional consequences of Prominin-1 modulation (if applicable, though not explicitly stated in abstract).
  • Characterization of Prominin-1 mRNA isoforms and their regulatory elements.

    • Main Results:

    • Prominin-1 is expressed on diverse stem cell types, including hematopoietic and endothelial progenitors.
    • The AC133 epitope is a well-established marker for isolating these progenitor cells.
    • The precise functional significance of Prominin-1 in stem cell biology requires further elucidation.

    Conclusions:

    • Prominin-1 is a cell-surface glycoprotein expressed on multiple stem cell types.
    • The AC133 epitope is a crucial tool for the isolation of hematopoietic and endothelial progenitor cells.
    • Further research is needed to fully understand the biological functions of Prominin-1 in stem cell populations.