Related Experiment Video

Updated: Aug 19, 2026

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
08:10

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Published on: May 2, 2019

Identification of genetic evidence for dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR

Manfred Weidmann1, P Schmidt, M Vackova

  • 1Unit for Microbiological Preparedness and Emerging Infectious Diseases, University Hospital, Stefan-Meier-Str. 19, 79014 Freiburg, Germany. manfred.weidmann@uniklinik-freiburg.de

Journal of Clinical Microbiology
|February 8, 2005
PubMed

More Related Videos

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR10:55

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Published on: November 10, 2018

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR07:20

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR

Published on: February 4, 2018

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...