Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic analysis of human glioblastomas using a genomic microarray system.

Tsuyoshi Suzuki1, Motohiko Maruno, Kouichi Wada

  • 1Department of Neurosurgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.

Brain Tumor Pathology
|February 9, 2005
PubMed
Summary

Genomic microarray analysis of glioblastomas identified frequent gene amplifications like RFC2/CYLN2 and deletions such as FGFR2. This technology aids in understanding brain tumor development and potential treatment responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoscopic Epidural Anterior Transpetrosal Approach (Endoscopic Kawase's Approach) for Meningiomas Around the Petrous Apex.

Operative neurosurgery (Hagerstown, Md.)·2026
Same author

Prediction of Overall Survival in Glioblastoma Using Early Postoperative Reduction in FLAIR Lesion Volume After Gross Total Resection.

Cancers·2026
Same author

Unexpected [18F]-fluciclovine hyperuptake in radiation necrosis after proton therapy: illustrative case.

Journal of neurosurgery. Case lessons·2026
Same author

Phase 1/2 study of a WT1 peptide-dosing emulsion in pediatric patients with recurrent/refractory diffuse intrinsic pontine glioma, glioblastoma, or anaplastic astrocytoma.

European journal of cancer (Oxford, England : 1990)·2026
Same author

Development and Validation of a Machine Learning Model to Predict Oral Anticoagulant Use in Stroke From Prothrombin Time-International Normalized Ratio and Activated Partial Thromboplastin Time.

Journal of the American Heart Association·2026
Same author

Molecular and clinical features of a Japanese medulloblastoma cohort: Subgroup-specific prognostic stratification using economical/accessible diagnostic methods.

Brain pathology (Zurich, Switzerland)·2026

Area of Science:

  • Oncology
  • Genetics
  • Genomics

Background:

  • Glioblastomas are aggressive primary brain tumors with complex genetic alterations.
  • Genomic microarray systems offer a high-throughput method for analyzing these alterations.

Purpose of the Study:

  • To characterize genome-wide gene amplifications, deletions, and chromosomal abnormalities in primary glioblastomas using the GenoSensor Array 300.
  • To identify molecular changes associated with glioblastoma development and potential clinical correlations.

Main Methods:

  • Analysis of genomic DNA from 30 frozen primary glioblastoma samples using the GenoSensor Array 300.
  • Comprehensive profiling of gene copy number variations and chromosomal aberrations.

Main Results:

Related Experiment Videos

  • Frequent gene amplifications observed for RFC2/CYLN2 (63.3%), EGFR (53.3%), and IL6 (53.3%).
  • Frequent gene deletions identified for FGFR2 (66.7%), MTAP (60.0%), and CDKN2A (p16)/MTAP (50.0%).
  • Common chromosomal gains included 7q (33.3%) and 7p (20.0%), while loss of 10q (46.7%) and chromosome 10 (30.0%) were frequent, often with EGFR amplification.

Conclusions:

  • The GenoSensor Array 300 is effective for identifying genome-wide molecular changes in glioblastomas.
  • Microarray profiles provide insights into brain tumor carcinogenesis and may correlate with treatment outcomes.