Related Experiment Video
Updated: Aug 19, 2026
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model
Published on: August 4, 2017
[Effects of adipose tissue extract on rat skin regeneration in vitro]
Gang Lu1, Xiao-bing Fu, Hai-hong Li
1Key Research Laboratory of Wound Repair, Burns Institute, 304th Clinical Department of General Hospital of PLA, Beijing 100037, China.
Objective:
To investigate the effects of adipose tissue extract on growth of rat skin in vitro.
Methods:
Neonatal rat skin (area of 2 mm x 2 mm) was cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% adipose tissue extract and 10% phosphate buffered solution (PBS). After being cultured for 6 days, the area of skin was measured.
Results:
Skin in both groups grew in area. With the addition of adipose tissue extract, the skin measured (8.43+/-1.40) mm2 in area after 6 days, and it was approximately 2 times of that of PBS group, in which the skin area was (4.18+/-1.11) mm2. The difference was statistically significant (P<0.05).
Conclusion:
Adipose tissue extract promote skin growth, which may be attributed to the presence of some growth factors in the extract.
