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[Study on multipotential differentiation of human bone marrow mesenchymal stem cells in vitro]
Ying Chang1, Xin Qi, Li-sha Bu
1Department of Neurology Sino-Japanese Friendship Hospital, Jilin university, Jilin 130031, Jilin, China.
Objective:
To study the capability of human bone marrow mesenchymal stem cells (MSCs) to differentiate into adipocyte, osteoblasts, and neurocytes under different experimental conditions in vitro.
Methods:
The induced human bone marrow MSCs were examined by cytochemistry staining and immunohistochemistry staining.
Results:
Induced human bone marrow MSCs formed alkaline phosphatase-positive aggregates and Von Kossa stain-positive nodules under the condition of osteogenic differentiation. Under the condition of adipogenic differentiation, the isolated human bone marrow MSCs formed oil red-O-positive cells. Immunohistochemistry results showed that differentiated human bone marrow MSCs expressed neuron-specific enolase (NSE) and neurofilament (NF-M).
Conclusion:
Human bone marrow MSCs have the capability of multipotential differentiation in vitro.