Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein and Protein Structure02:15

Protein and Protein Structure

88.6K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
88.6K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.6K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.6K
What are Proteins?01:55

What are Proteins?

240.1K
Overview
240.1K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Families02:47

Protein Families

17.1K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Redesign of energetically frustrated regions rescues function in defective T4 clamp loaders.

bioRxiv : the preprint server for biology·2026
Same author

The scientific legacy of Martin Karplus from the perspective of his collaborators.

Biophysical journal·2026
Same author

BTK autoinhibition analyzed by high-throughput swaps of SH2 domains.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Autophosphorylation of oncoprotein TEL-ABL in myeloid and lymphoid cells confers resistance to the allosteric ABL inhibitor asciminib.

Science signaling·2025
Same author

Neuraminidase 1 secondary deficiency contributes to CNS pathology in neurological mucopolysaccharidoses via brain protein hypersialylation.

The Journal of clinical investigation·2025
Same author

Thiophosphate bioisosteres of inositol hexakisphosphate enhance binding affinity and residence time on bacterial virulence factors.

RSC chemical biology·2025

Related Experiment Video

Updated: Feb 11, 2026

Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.9K

SAXS and the working protein.

Bhushan Nagar, John Kuriyan

    Structure (London, England : 1993)
    |February 9, 2005
    PubMed
    Summary

    Shape reconstructions of the molecular motor p97 reveal how ATP consumption drives cyclical domain motions. Small angle X-ray scattering (SAXS) offers new ways to visualize biological machines in solution.

    Area of Science:

    • Structural Biology
    • Biophysics
    • Biochemistry

    Background:

    • The molecular motor p97 plays a crucial role in cellular processes.
    • Understanding the mechanism of ATP consumption in molecular motors is essential.

    Discussion:

    • Davies et al. utilized small angle X-ray scattering (SAXS) to reconstruct the shape of the p97 molecular motor.
    • The study provides insights into the coupling of ATP consumption with cyclical domain motions within p97.

    Key Insights:

    • SAXS is a powerful technique for visualizing the dynamic behavior of biological machines in solution.
    • The research elucidates the functional mechanism of p97, linking its structure to its ATP-dependent activity.

    Outlook:

    • Further application of SAXS can reveal the workings of other complex biological machines.

    More Related Videos

    Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
    09:01

    Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

    Published on: June 26, 2020

    7.6K
    Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
    11:09

    Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

    Published on: January 5, 2017

    18.0K

    Related Experiment Videos

    Last Updated: Feb 11, 2026

    Analysis of SEC-SAXS data via EFA deconvolution and Scatter
    10:59

    Analysis of SEC-SAXS data via EFA deconvolution and Scatter

    Published on: January 28, 2021

    9.9K
    Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
    09:01

    Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

    Published on: June 26, 2020

    7.6K
    Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
    11:09

    Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

    Published on: January 5, 2017

    18.0K
  • This study paves the way for understanding molecular motor function at a higher resolution.