A cultural renaissance: in vitro cell biology embraces three-dimensional context

Increasingly, researchers are recognizing the limitations of two-dimensional (2-D), monolayer cell culture and embracing more realistic three-dimensional (3-D) cell culture systems. Currently, 3-D culture techniques are being employed by neuroscientists to grow cells from the central nervous system. From this work, it has become clear that 3-D cell culture offers a more realistic milieu in which the functional properties of neurons can be observed and manipulated in a manner that is not possible in vivo. The implications of this technical renaissance in cell culture for both clinical and basic neuroscience are significant and far-reaching.