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Updated: Aug 19, 2026
Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
Published on: January 28, 2015
[Induced differentiation of human cord blood monocytes into neuron-like cells in vitro]
Tai-Yun Liu1, Cheng Zhang, Lu-Lu Xiao
1Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510089, China. ltylxlf@gzsums.edu.cn
Objective:
To probe the conditions for inducing human cord blood monocytes to differentiate into neuron-like cells.
Methods:
The mononuclear cells were isolated from human umbilical cord blood samples and plated in 25-mm culture flasks containing DMEM/F12 medium. The fifth passage of mesenchymal stem cells (MSCs) were induced by beta-mercaptoe- thanol (beta-ME), dimethyl sulfoxide (DMSO) and conditioned medium for neuron induction, respectively, to differentiate into neuron-like cells. The expression of nestin, neuron-specific enolase (NSE) and neurofilament (NF) on the treated cells were detected by immunocytochemical method.
Results:
Nestin expression were found in 6.7% of the primary, 12.4% of the second passage, 20.8% of the fifth passage of MSCs, respectively. Thirty-six percent of cell cultures treated with the conditioned medium for neuron induction were immunoreactive for NSE and NF, and 33% of the cells induced by beta-ME and 25% of the cells by DMSO also expressed NSE and NF.
Conclusion:
Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.
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