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Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
Published on: September 1, 2019
[Effect of 32P irradiation on cell cycle of HL-60 cells]
1Center of Analysis, Guangdong Medical College, Zhanjiang 524023, China.
Objective:
To investigate the effect of (32)P irradiation on the cell cycle of HL-60 cells.
Methods:
Flow cytometry was employed to detect cell cycle changes of HL-60 cells induced by (32)P irradiation at different doses for varied time lengths (24, 28 and 72 h, respectively).
Result:
(32)P could induce cell cycle arrest in S and G(2)/M phases, and the arrest occurred initially in G(2)/M phase.
Conclusion:
(32)P may significantly interfere with the cell cycle of HL-60 cells, causing cell cycle arrest primarily in G(2)/M.
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