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Improved synthesis and rapid isolation millimole quantities of adenylysulfate
Zeitschrift Fur Naturforschung. Section C, Biosciences
|May 1, 1979
Abstract:
An improved enzymatic method for the synthesis of adenylysulfate (APS) from adenosine 5'-phosphate using APS-reductase from Thiobacillus denitrificans is described. Isolation of millimole quantitities of this sulfur nucleotide is achieved rapidly by means of ion exchange chromatography on a strongly basic ion exchange resin. A facile and reproducible desalting procedure is described.