Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

H-2RIIBP expressed from a baculovirus vector binds to multiple hormone response elements.

M S Marks1, B Z Levi, J H Segars

  • 1Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

Molecular Endocrinology (Baltimore, Md.)
|February 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of non-genomic androgen signalling in suppressing proliferation of fibroblasts and fibrosarcoma cells.

Cell death & disease·2014
Same author

The pluripotency factor nanog promotes breast cancer tumorigenesis and metastasis.

Oncogene·2013
Same author

The impact of uterine leiomyomas on reproductive outcomes.

Minerva ginecologica·2010
Same author

Transfer of antibodies elicited by baculovirus-derived VP2 of a very virulent bursal disease virus strain to progeny of commercial breeder chickens.

Avian pathology : journal of the W.V.P.A·2009
Same author

PML enhances the regulation of p53 by CK1 in response to DNA damage.

Oncogene·2008
Same author

Inhibition of the SH3 domain-mediated binding of Src to the androgen receptor and its effect on tumor growth.

Oncogene·2007

H-2RIIBP, a nuclear hormone receptor, binds to major histocompatibility complex class I genes and various hormone response elements. This suggests H-2RIIBP may have multiple functions in development and gene regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • H-2RIIBP is a nuclear hormone receptor superfamily member.
  • It binds to the region II enhancer of major histocompatibility complex class I genes.
  • Homology with Drosophila XR2C/CF1 suggests a role in development.

Purpose of the Study:

  • To produce recombinant H-2RIIBP and characterize its DNA-binding specificity.
  • To investigate H-2RIIBP's interaction with various hormone response elements.
  • To determine the potential pleiotropic function of H-2RIIBP.

Main Methods:

  • Baculovirus expression system for recombinant H-2RIIBP production.
  • Generation of monoclonal antibodies against H-2RIIBP.
  • DNA-protein immunoprecipitation, Southwestern, and gel mobility shift assays.

Related Experiment Videos

  • Methylation interference assays to identify DNA-binding motif.

    • Main Results:

    • Recombinant H-2RIIBP was successfully produced and localized to the nucleus.
    • H-2RIIBP binds to estrogen response elements with high affinity.
    • It also binds to some thyroid hormone and retinoic acid response elements with lower affinity.
    • The conserved hormone response motif GG(T/A)CA was identified as the H-2RIIBP binding site.

    Conclusions:

    • H-2RIIBP binds to a variety of hormone response elements.
    • This binding occurs without exogenous ligand addition.
    • H-2RIIBP likely possesses a pleiotropic function, impacting multiple cellular processes.