Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Size01:22

Cell Size

Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.Surface AreaCells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding the cells limits the...
Cell Diversity01:13

Cell Diversity

The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular organisms...
Capillaries and Their Types01:20

Capillaries and Their Types

Capillaries, a crucial constituent of the circulatory system, are diminutive vessels with a diameter between 5–10 micrometers, accommodating perfusion to the tissues through the phenomenon known as microcirculation. Through their permeable walls, consisting of an endothelial layer ensconced by a basement membrane and sporadically dispersed smooth muscle fibers, the exchange of substances between the blood and the interstitial fluid becomes plausible. Variance in wall composition exists, with...
Microbial Morphologies01:29

Microbial Morphologies

Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
Diversity of Protists II01:27

Diversity of Protists II

Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a pressure hemostatic dressing with the coagulation properties of sodium alginate as a base material.

Journal of applied biomaterials & functional materials·2026
Same author

Risk Factors for Free Flap Failure in Head and Neck Reconstruction: A Retrospective Analysis From a Single Medical Center.

Plastic and reconstructive surgery. Global open·2026
Same author

Integrative gene ontology-driven analysis of the eutopic endometrium reveals key dysregulated functionomes and pathways in endometriosis.

Journal of the Chinese Medical Association : JCMA·2026
Same author

Evaluating Thiram-Induced Embryotoxicity Using Integrated In Silico, In Vitro, and Transcriptomic Approaches.

Environmental toxicology·2026
Same author

Refinement of maximal levator resection for blepharoptosis correction: High incision and advancement of levator complex.

JPRAS open·2026
Same author

Evaluation of the Effectiveness of Immediate Preoperative Team Discussion in Enhancing Surgical Safety and Clinical Outcomes in Head and Neck Free Flap Reconstruction.

Microsurgery·2026

Related Experiment Video

Updated: Jul 15, 2026

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
07:26

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

Published on: December 5, 2019

Biocompatible micropatterning of two different cell types.

Carlos C Co1, Yu-Chi Wang, Chia-Chi Ho

  • 1Department of Chemical and Materials Engineering, University of Cincinnati, 497 Rhodes Hall, Cincinnati, OH 45221, USA.

Journal of the American Chemical Society
|February 11, 2005
PubMed
Summary

This study introduces a novel polyelectrolyte assembly method for non-cytotoxic micropatterning of multiple cell types. This technique enables complex tissue engineering by precisely arranging endothelial cells and fibroblasts on biodegradable substrates.

More Related Videos

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
08:59

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps

Published on: October 28, 2018

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Related Experiment Videos

Last Updated: Jul 15, 2026

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
07:26

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

Published on: December 5, 2019

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
08:59

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps

Published on: October 28, 2018

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Micropatterning techniques using soft-lithography enable control over cell arrangement.
  • Existing methods face challenges in non-cytotoxic conversion of cell-resistant to cell-adhesive regions for multi-cell type patterning.
  • Recreating complex tissue structures in vitro remains a significant hurdle in tissue engineering.

Purpose of the Study:

  • To present a novel polyelectrolyte assembly approach for non-cytotoxic micropatterning of multiple cell types.
  • To demonstrate the ability to sequentially pattern different cell types on biodegradable substrates.
  • To showcase the application of this method in creating organized cellular structures, such as capillary networks.

Main Methods:

  • Utilized soft-lithography for initial micropatterning.
  • Employed a polyelectrolyte assembly technique for converting cell-resistant regions to cell-adhesive regions.
  • Applied the method to pattern endothelial cells and fibroblasts on biodegradable substrates.
  • Induced capillary formation by endothelial cells and subsequent fibroblast assembly.

Main Results:

  • Successfully achieved non-cytotoxic conversion of micropatterned regions.
  • Demonstrated successful sequential micropatterning of two distinct cell types (endothelial cells and fibroblasts).
  • Showcased organized capillary formation by endothelial cells on micropatterned lines.
  • Facilitated subsequent assembly of fibroblasts around the patterned endothelial cells.

Conclusions:

  • The polyelectrolyte assembly approach offers a versatile and non-cytotoxic solution for multi-cell type micropatterning.
  • This method advances tissue engineering capabilities for in vitro reconstruction of complex tissue architectures.
  • The technique holds promise for applications requiring precise spatial control of multiple cell types, including vascular tissue engineering.