Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interfacial pH at an isolated silica-water surface.

Josephine P O'Reilly1, Craig P Butts, Ian A l'Anson

  • 1School of Biological and Chemical Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK.

Journal of the American Chemical Society
|February 11, 2005
PubMed
Summary

Measuring interfacial pH with a pH-sensitive dye tethered to silica reveals a surface pH 2 units lower than the bulk. This occurs due to attracted H+ ions concentrating near the surface potential.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caylobolide B: Structure Revision, Total Synthesis, Biological Characterization, and Discovery of New Analogues.

Angewandte Chemie (International ed. in English)·2025
Same author

Mass-Standardised Differential Antibody Binding to a Spectrum of SARS-CoV-2 Variant Spike Proteins: Wuhan, Alpha, Beta, Gamma, Delta, Omicron BA.1, BA.4/5, BA.2.75 and BA.2.12.1 Variants-Antibody Immunity Endotypes.

Immunology·2025
Same author

The combination of synthesis and ultra-high-resolution NMR spectroscopy reveals the correct structure of caylobolide A.

Nature synthesis·2025
Same author

A validated experimental NMR parameter dataset of organic molecules to assist benchmarking of 3D structure determination methods.

The Analyst·2025
Same author

IMPRESSION generation 2 - accurate, fast and generalised neural network model for predicting NMR parameters in place of DFT.

Chemical science·2025
Same author

Crystal Structure and NMR of an α,δ-Peptide Foldamer Helix Shows Side-Chains are Well Placed for Bifunctional Catalysis: Application as a Minimalist Aldolase Mimic.

Angewandte Chemie (Weinheim an der Bergstrasse, Germany)·2024

Area of Science:

  • Surface Chemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Interfacial pH measurements are crucial for understanding surface reactions.
  • Silica surfaces exhibit significant surface potential influencing local ion concentrations.
  • Optical methods offer non-invasive approaches to probe interfacial environments.

Purpose of the Study:

  • To optically measure the pH at the interface of a silica surface.
  • To investigate the relationship between surface potential and interfacial pH.
  • To determine if bulk pH changes affect the established near-surface pH layer.

Main Methods:

  • Tethering a pH-sensitive dye to a silica surface.
  • Utilizing optical measurements to determine interfacial pH.

Related Experiment Videos

  • Comparing interfacial pH with bulk pH measurements.

    • Main Results:

    • The interfacial pH was found to be approximately 2 pH units lower than the bulk pH.
    • An enhanced concentration of H+ ions at the interface was observed, consistent with a negative surface potential (-120 mV).
    • The near-surface charged layer proved stable and unaffected by bulk pH variations.

    Conclusions:

    • Optical measurement of interfacial pH using dye-functionalized silica is feasible.
    • Silica surfaces create a localized acidic environment due to surface potential.
    • The interfacial pH layer is robust and independent of bulk solution conditions.