Related Experiment Video
Updated: Aug 3, 2026
Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging
Published on: May 11, 2014
Detection of nonpigmented endometriotic lesions with 5-aminolevulinic acid-induced fluorescence
Olaf Buchweitz1, Pia Wülfing, Anette Staebler
1Department of Obstetrics and Gynecology, University of Münster, Albert Schweitzer Strasse 33, D-48149 Münster, Germany.
Study Objective:
To evaluate the feasibility of fluorescence diagnosis of nonpigmented (red and white) endometriotic lesions with 20 mg/kg of 5-aminolevulinc acid (5-ALA) 5-7 and 10-14 hours before surgery.
Design:
Prospective analysis (Canadian Task Force classification II-2).
Setting:
University hospital.
Patients:
Twenty-four consecutive patients with suspected endometriosis undergoing laparoscopy.
Intervention:
Laparoscopic surgery under white light illumination and fluorescence diagnosis.
Measurements And Main Results:
The total number of endometriotic lesions detected under white light illumination was compared with the number of lesions detected with fluorescence diagnosis. Fluorescence diagnosis yielded an overall improvement of 35% in the detection of nonpigmented endometriotic lesions compared with white light illumination. Sensitivity (91%) and specificity (79%) were similar 5-7 and 10-14 hours before surgery.
Conclusion:
The dosage of 20 mg/kg body weight of 5-ALA is feasible for fluorescence diagnosis of nonpigmented endometriosis. Sensitivity of fluorescence diagnosis with 20 mg/kg is similar to that achieved with a 30-mg/kg dose. Sensitivity does not change within the application period 5-7 and 10-14 hours before surgery.
