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[Determination of ivermectin in pig serum by high performance liquid chromatography]
Jian-hong Zhao1, Cheng-jun Sun, Li-sha Mao
1Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To establish a high performance liquid chromatographic method for the determination of micro-amount of ivermectin in pig serum.
Methods:
Ivermectin in pig serum was extracted with ethyl acetate after the serum protein was precipitated with 0.05% metaphosphoric acid-methanol in the ratio of 7:3 (V/V). Then the sample was centrifuged at 4000 r/min for 5 min and the supernate was evaporated to dryness with rotary vacuum evaporator. The residue was dissolved with 0.20 ml of methanol as the sample solution for HPLC analysis. HPLC column used was a phenomenex C18 (5 microm, 250 mm x 4.6 mm) with a same type of guard column. Mobile phase consisted of methanol and water in the ratio of 90:10 (V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 245 nm.
Results:
The linear range of the method was found to be 0.010-20 mg/L and its detection limit was 0.010 mg/L. The relative standard deviation of the method was 0.78%-3.82% and the recoveries varied from 94.0% to 100.0%, with an average recovery of 97.3%.
Conclusion:
This method is simple, reproducible, accurate and suitable to the determination of micro-amount of ivermectin in pig serum.
