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Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
Published on: July 13, 2017
Complete sequence and gene organization of the Nosema spodopterae rRNA gene
Shu-Jen Tsai1, Wei-Fone Huang, Chung-Hsiung Wang
1Department of Entomology, National Taiwan University, Taipei 106, Taiwan.
Abstract:
By sequencing the entire ribosomal RNA (rRNA) gene of Nosema spodopterae, we show here that its gene organization follows a pattern similar to the Nosema type species, Nosema bombycis, i.e. 5'-large subunit rRNA (2,497 bp)-internal transcribed spacer (185 bp)-small subunit rRNA (1,232 bp)-intergenic spacer (277 bp)-5S rRNA (114 bp)-3'. Gene sequences and the secondary structures of large subunit rRNA, small subunit rRNA, and 5S rRNA are compared with the known corresponding sequences and structures of closely related microsporidia. The results suggest that the Nosema genus may be heterogeneous and that the rRNA gene organization may be a useful characteristic for determining which species are closely related to the type species.
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