Related Experiment Video

Updated: Aug 19, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

DJ-1 restores p53 transcription activity inhibited by Topors/p53BP3

Yumi Shinbo1, Takahiro Taira, Takeshi Niki

  • 1Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

International Journal of Oncology
|February 11, 2005
PubMed

Insights

DJ-1 protein interacts with Topors and p53, influencing p53

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Neuroscience

Background:

  • DJ-1 is a multifunctional protein implicated in Parkinson's disease pathogenesis.
  • DJ-1's roles in transcriptional regulation and oxidative stress response are established.
  • Topors (p53BP3) is a ring finger protein interacting with topoisomerase I and p53.

Purpose of the Study:

  • To investigate the interaction between DJ-1 and Topors.
  • To elucidate the role of DJ-1 in regulating p53 activity.
  • To understand the mechanism of DJ-1's influence on p53 sumoylation.

Main Methods:

  • In vitro and in vivo binding assays.
  • Cellular colocalization studies.
  • UV irradiation to stimulate protein interactions.
  • Analysis of p53 transcriptional activity and sumoylation levels.

Main Results:

  • DJ-1 binds to Topors and p53 both in vitro and in vivo.
  • DJ-1 and Topors colocalize within cells.
  • Topors mediates sumoylation of DJ-1 and p53.
  • UV irradiation enhances DJ-1 and p53 binding and colocalization.
  • Topors abrogates p53 transcriptional activity via sumoylation.
  • DJ-1 restores repressed p53 activity by releasing sumoylated p53.

Conclusions:

  • DJ-1 positively regulates p53 transcriptional activity.
  • This regulation occurs through Topors-mediated sumoylation.
  • DJ-1's function in p53 regulation may be relevant to Parkinson's disease.

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