[Optimized RT-PCR based detection of specific genetic abnormalities within malignant hematopoietic disorders]

The moleculargenetic detection of specific genetic abnormalities within malignant hematopoietic disorders is an important diagnostic tool with relevance for the differential diagnosis, therapy and prognosis.According to a modified and optimized RT-PCR based technique native bone marrow aspirates and peripheral blood samples of 183 patients were investigated for the presence of specific genetic abnormalities. The histological diagnosis of all CMLs t(9;22) and variants of AMLs M3 t(15;17) and t(11;17) were moleculargenetically confirmed. Additionally one t(5;12) positive chronic myelomonozytic leukemia, one t(8;21) positive AML M1, one t(9;22) positive B-ALL and in each case one t(6;9) and one t(3;21) positive myelodysplastic syndrome were detected. The hereby described method is a simple, specific and reliable technique for the rapid moleculargenetic detection of specific genetic abnormalities within malignant hematopoietic disorders with implication for the diagnosis/differential diagnosis, prognosis and therapy.